Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Brevundimonas diminuta | |
5-Hydroxyisoquinoline | rapid and very effective inactivation in presence as well as in absence of the electron acceptors iodonitrotetrazolium chloride, phenazine methosulfate or ferricyanide | Brevundimonas diminuta | |
arsenite | - |
Brevundimonas diminuta | |
CN- | - |
Brevundimonas diminuta | |
Isoquinoline | slow inhibition in absence of an electron acceptor | Brevundimonas diminuta | |
methanol | - |
Brevundimonas diminuta | |
p-hydroxymercuribenzoate | - |
Brevundimonas diminuta |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | enzyme contains 3.95 g-atom of iron per mol of enzyme. The iron and the acid-labile sulfur are arranged in two (2Fe-2S) clusters | Brevundimonas diminuta | |
Mo | enzyme contains 0.85 g-atom of molybdenum per mol of enzyme | Brevundimonas diminuta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
16000 | - |
1 * 80000 + 1 * 16000, SDS-PAGE | Brevundimonas diminuta |
80000 | - |
1 * 80000 + 1 * 16000, SDS-PAGE | Brevundimonas diminuta |
93000 | 95000 | gel filtration, analytical ultracentrifugation | Brevundimonas diminuta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Isoquinoline + acceptor + H2O | Brevundimonas diminuta | first enzyme of the isoquinoline catabolism | ? | - |
? | |
Isoquinoline + acceptor + H2O | Brevundimonas diminuta 7 | first enzyme of the isoquinoline catabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevundimonas diminuta | - |
- |
- |
Brevundimonas diminuta 7 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brevundimonas diminuta |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.87 | - |
- |
Brevundimonas diminuta |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxyisoquinoline + acceptor + H2O | - |
Brevundimonas diminuta | 1,5-dihydroisoquinoline + reduced acceptor | - |
? | |
5-hydroxyisoquinoline + acceptor + H2O | - |
Brevundimonas diminuta 7 | 1,5-dihydroisoquinoline + reduced acceptor | - |
? | |
isoquinoline + acceptor + H2O | acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone | Brevundimonas diminuta | 1-oxo-1,2-dihydroisoquinoline + reduced acceptor | - |
? | |
isoquinoline + acceptor + H2O | acceptors: 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-phenyl-tetrazolium chloride, formazan, medola's blue, N-methyldibenzylpyrazine methylsulfate, nitroblue tetrazolium chloride, cytochrome c, ferricyanide, 1,2-benzoquinone | Brevundimonas diminuta 7 | 1-oxo-1,2-dihydroisoquinoline + reduced acceptor | - |
? | |
Isoquinoline + acceptor + H2O | first enzyme of the isoquinoline catabolism | Brevundimonas diminuta | ? | - |
? | |
Isoquinoline + acceptor + H2O | first enzyme of the isoquinoline catabolism | Brevundimonas diminuta 7 | ? | - |
? | |
phthalazine + acceptor + H2O | at 89% of the activity compared to isoquinoline | Brevundimonas diminuta | 1-oxophthalazine + reduced acceptor | - |
? | |
phthalazine + acceptor + H2O | at 89% of the activity compared to isoquinoline | Brevundimonas diminuta 7 | 1-oxophthalazine + reduced acceptor | - |
? | |
quinazoline + acceptor + H2O | at 45% of the activity compared to isoquinoline | Brevundimonas diminuta | 4-hydroxyquinazoline + reduced acceptor | - |
? | |
quinazoline + acceptor + H2O | at 45% of the activity compared to isoquinoline | Brevundimonas diminuta 7 | 4-hydroxyquinazoline + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 80000 + 1 * 16000, SDS-PAGE | Brevundimonas diminuta |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 60 | - |
Brevundimonas diminuta |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
5 min, rapid decrease of activity above | Brevundimonas diminuta |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Brevundimonas diminuta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme contains no FAD | Brevundimonas diminuta | |
Pterin molybdenum cofactor | - |
Brevundimonas diminuta |